Breaking the Limits of Vision: Super-Resolution Microscopy Achieves Nanometer Precision Without ON/OFF Switching
Breaking the Limits of Vision: Super-Resolution Microscopy Achieves Nanometer Precision Without ON/OFF Switching 1. Introduction Super-resolution microscopy has revolutionized imaging at the nanoscale, enabling researchers to observe molecular and cellular structures with unprecedented clarity. A recent breakthrough allows for achieving nanometer resolution without the need for traditional ON/OFF switching of fluorescent molecules, simplifying the imaging process and increasing efficiency. 2. The Challenge with Traditional Super-Resolution Microscopy Conventional techniques like STORM (Stochastic Optical Reconstruction Microscopy) and PALM (Photoactivated Localization Microscopy) rely on switching fluorescent molecules ON and OFF. This process requires specialized fluorophores and complex illumination setups. ON/OFF switching can introduce errors and requires precise control to prevent photobleaching. 3. The Revolutionary Approach The new method eliminates the need for ON/OFF switching...